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dapi nuclear staining  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology dapi nuclear staining
    Dapi Nuclear Staining, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+dapi/DAPI/pm42869916-314-14-20
    Average 96 stars, based on 1459 article reviews
    dapi nuclear staining - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Single-nucleus multiomics of murine gonads reveals transcriptional regulatory network underlying supporting lineage differentiation
    Article Snippet: Specifically, RNAscope probes Mm-Lef1 (catalog no. 441861) and Mm-Msx1 (catalog no. 421841, Advanced Cell Diagnostics) were applied. .. Before 4′,6-diamidino-2-phenylindole (DAPI) staining, the slides were blocked in blocking buffer (5% donkey serum/0.1% Triton X-100 in PBS) for 1 hour at room temperature and counterstained with anti–COUP-TFII (1:200, R&D Systems, catalog no. PP-H7147-00), anti-AMH (1:500, Santa Cruz Biotechnology, catalog no. sc-6886), or anti-FOXL2 (1:200, Novus Biological, catalog no. NB100-1277) antibodies diluted in blocking buffer overnight at 4°C. .. The next day, the slides were washed and incubated with donkey anti-mouse (1:200, Invitrogen, catalog no. A10037) and donkey anti-goat (1:200, Invitrogen, catalog no. A21447) secondary antibodies diluted in blocking buffer incubation for 1 hour at room temperature.

    Article Title: CD200 + fibroblasts prime the immunoregulatory function of granulocytic myeloid-derived suppressor cells in inflammatory arthritis.
    Article Snippet: .. Secondary antibodies were stained for 1 h at room temperature followed by incubation with DAPI nuclear staining (1:1000, Cat# sc-3598, Santa Cruz). .. The following antibodies were used in human tissues: anti-CD33 (Polyclonal, 1:100, Cat# bs-2042R, Bioss), Nature Communications | (2026) 17:10746 12 anti-HLA-DR (LN3, 1:100, Cat# LS-B8114, LSBio), anti-CD20 (H1, 1:500, Cat# 555677, BD Biosciences), anti-CD56 (123A8, 1:1000, Cat# NBP244713, Novus), anti-CD11c (Polyclonal, 1:300, Cat# orb13554, Biorbyt), anti-CD3 (PS1, 1:50, Cat# ab699, Abcam), anti-CD14 (4B4F12, 1:100, Cat# ab182032, Abcam), anti-CD16 (2Q1240, 1:50, Cat# sc-70548, Santa Cruz), anti-CD123 (6H6, 1:100, Cat# 306002, BioLegend), anti-CD274/ PD-L1 (E1L3N, 1:100, Cat# 85164SF, Cell Signaling), anti-CD200 (Polyclonal, 1:100, Cat#AF2724, R&DSystems), anti-Podoplanin (Polyclonal, 1:100, Cat# AF3670, R&D Systems).

    Article Title: A cell-type-resolved human brain atlas of microRNAs and tRNA fragments
    Article Snippet: Cells were pelleted by centrifugation at 900 g for 5 minutes, resuspended in staining buffer, and incubated for 30 minutes with secondary antibodies for neurons (1:500; Alexa FluorTM 647 conjugated, Jackson ImmunoResearch, 711-605-152) and astrocytes (1:500; Cy3-conjugated; Jackson ImmunoResearch, 115-165-072). .. Cells were pelleted again as above and resuspended in staining buffer containing DAPI (1μg/mL; Santa Cruz, sc-3598). ..

    Article Title: Macrophage-derived IL-6 reprograms lipid metabolism to promote colorectal cancer development through USP14-mediated FASN deubiquitination
    Article Snippet: For immunohistochemistry, the slices were incubated with antibodies against IL-6 (Cat#AF-206-NA, R&D Systems, Minneapolis, Minnesota, USA), p-STAT3 (Cell Signaling Technology, Danvers, Massachusetts, USA), USP14 (Proteintech, Wuhan, Hubei, China) and FASN (Cat#10624-2-AP, Proteintech) and then treated with an HRP-labeled secondary antibody (Proteintech). .. After the final round of staining, DAPI (Cat#sc-3598, Santa Cruz Biotechnology, Texas, USA) solution was added. .. Photomicrographs were obtained via a light microscope (Leica, Germany).

    Article Title: Arabinoxylan derived from corn bran by-product: structural characterization and anti-inflammatory activity
    Article Snippet: 1 College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China 2 Key Laboratory of Traditional Chinese Korean Medicine Research (Yanbian University) of State Ethnic Affairs Commission, College of Pharmacy, Yanbian University, Yanji 133002, China 3 National Engineering Research Center for Wheat and Corn Deep Processing, Jilin Agricultural University, Changchun 130118, China Abstract Arabinoxylan, the preeminent constituent of hemicellulose B present in cereal bran, is widely recognized for its myriad physiological benefits, encompassing antioxidant, anticarcinogenic, immunomodulatory, intestinal protective, and prebiotic activities, thereby underscoring its significance in promoting health and wellness.. Recent research extracted arabinoxylan (AX) from corn bran and fractionated it into AX-1–1, AX-2–1, and AX-3–1, which were purified and structurally analyzed.. These fractions had average molecular weights of 287, 305, and 280 kDa, respectively, and consisted mainly of arabinose and xylose, along with other saccharides, with A/X ratios of 0.78, 1.02, and 0.92.

    Article Title: Glycine receptors in circulating white blood cells regulated by neuroinflammation
    Article Snippet: After incubation, the slides were washed three times with PBS for 5 minutes each, then incubated with appropriate secondary antibodies, i.e., Alexa Fluor 488 (1:200) and/or 594 (1:200) (Thermo-Fisher Scientific) for 1 hour at room temperature. .. A DAPI mounting agent (# AB104139 , Santa Cruz Biotechnology) was used for nuclear staining and mounting. ..

    Article Title: Oral KDS2010, a Monoamine Oxidase-B (MAO-B) Inhibitor, Slows the Deterioration of Motor Coordination in Genetic SCA1 Models by Inhibiting Astrocytic MAO-B-Mediated Inflammation.
    Article Snippet: The slides were incubated at 4°C for 24 h. After incubation, the slides were washed again with TBS buffer containing Triton X- 100 and incubated with the appropriate secondary antibodies (Anti- Rat IgG Alexa Fluor 488 (Invitrogen, cat. no. A21208), Anti- Rat IgG Alexa Fluor 633 (Invitrogen, cat. no. A21070), Anti- Mouse IgG Alexa Fluor 555 (Invitrogen, cat. no. A32727) and Anti- Rabbit IgG Alexa Fluor 568 (Invitrogen, cat. no. A- 11011)). .. During the third washing step, add DAPI (4′,6- diamidino- 2- phenylindole) (Santa Cruz, cat. no. sc- 373 715) to stain the nuclei, then mount the slides for imaging. .. During the third washing step, add DAPI (4′,6- diamidino- 2- phenylindole) (Santa Cruz, cat. no. sc- 373 715) to stain the nuclei, then mount the slides for imaging.

    Blocking Assay:

    Article Title: Single-nucleus multiomics of murine gonads reveals transcriptional regulatory network underlying supporting lineage differentiation
    Article Snippet: Specifically, RNAscope probes Mm-Lef1 (catalog no. 441861) and Mm-Msx1 (catalog no. 421841, Advanced Cell Diagnostics) were applied. .. Before 4′,6-diamidino-2-phenylindole (DAPI) staining, the slides were blocked in blocking buffer (5% donkey serum/0.1% Triton X-100 in PBS) for 1 hour at room temperature and counterstained with anti–COUP-TFII (1:200, R&D Systems, catalog no. PP-H7147-00), anti-AMH (1:500, Santa Cruz Biotechnology, catalog no. sc-6886), or anti-FOXL2 (1:200, Novus Biological, catalog no. NB100-1277) antibodies diluted in blocking buffer overnight at 4°C. .. The next day, the slides were washed and incubated with donkey anti-mouse (1:200, Invitrogen, catalog no. A10037) and donkey anti-goat (1:200, Invitrogen, catalog no. A21447) secondary antibodies diluted in blocking buffer incubation for 1 hour at room temperature.

    Incubation:

    Article Title: CD200 + fibroblasts prime the immunoregulatory function of granulocytic myeloid-derived suppressor cells in inflammatory arthritis.
    Article Snippet: .. Secondary antibodies were stained for 1 h at room temperature followed by incubation with DAPI nuclear staining (1:1000, Cat# sc-3598, Santa Cruz). .. The following antibodies were used in human tissues: anti-CD33 (Polyclonal, 1:100, Cat# bs-2042R, Bioss), Nature Communications | (2026) 17:10746 12 anti-HLA-DR (LN3, 1:100, Cat# LS-B8114, LSBio), anti-CD20 (H1, 1:500, Cat# 555677, BD Biosciences), anti-CD56 (123A8, 1:1000, Cat# NBP244713, Novus), anti-CD11c (Polyclonal, 1:300, Cat# orb13554, Biorbyt), anti-CD3 (PS1, 1:50, Cat# ab699, Abcam), anti-CD14 (4B4F12, 1:100, Cat# ab182032, Abcam), anti-CD16 (2Q1240, 1:50, Cat# sc-70548, Santa Cruz), anti-CD123 (6H6, 1:100, Cat# 306002, BioLegend), anti-CD274/ PD-L1 (E1L3N, 1:100, Cat# 85164SF, Cell Signaling), anti-CD200 (Polyclonal, 1:100, Cat#AF2724, R&DSystems), anti-Podoplanin (Polyclonal, 1:100, Cat# AF3670, R&D Systems).

    Article Title: Neuroprotective Effects of Strength Training on Behavioral Deficit, Oxidative Damage, Astrogliosis, and Neuronal Death in a Bipolar Disorder Model
    Article Snippet: .. Sections were then incubated for 1 h in a solution containing 0.01% FJC (MilliporeSigma; cat. no. AG325) and 0.01% DAPI (Santa Cruz Biotechnology; cat. no. sc‐3598) in 0.1% acetic acid. ..

    Fluorescence:

    Article Title: Arabinoxylan derived from corn bran by-product: structural characterization and anti-inflammatory activity
    Article Snippet: 1 College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China 2 Key Laboratory of Traditional Chinese Korean Medicine Research (Yanbian University) of State Ethnic Affairs Commission, College of Pharmacy, Yanbian University, Yanji 133002, China 3 National Engineering Research Center for Wheat and Corn Deep Processing, Jilin Agricultural University, Changchun 130118, China Abstract Arabinoxylan, the preeminent constituent of hemicellulose B present in cereal bran, is widely recognized for its myriad physiological benefits, encompassing antioxidant, anticarcinogenic, immunomodulatory, intestinal protective, and prebiotic activities, thereby underscoring its significance in promoting health and wellness.. Recent research extracted arabinoxylan (AX) from corn bran and fractionated it into AX-1–1, AX-2–1, and AX-3–1, which were purified and structurally analyzed.. These fractions had average molecular weights of 287, 305, and 280 kDa, respectively, and consisted mainly of arabinose and xylose, along with other saccharides, with A/X ratios of 0.78, 1.02, and 0.92.

    Microscopy:

    Article Title: Arabinoxylan derived from corn bran by-product: structural characterization and anti-inflammatory activity
    Article Snippet: 1 College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China 2 Key Laboratory of Traditional Chinese Korean Medicine Research (Yanbian University) of State Ethnic Affairs Commission, College of Pharmacy, Yanbian University, Yanji 133002, China 3 National Engineering Research Center for Wheat and Corn Deep Processing, Jilin Agricultural University, Changchun 130118, China Abstract Arabinoxylan, the preeminent constituent of hemicellulose B present in cereal bran, is widely recognized for its myriad physiological benefits, encompassing antioxidant, anticarcinogenic, immunomodulatory, intestinal protective, and prebiotic activities, thereby underscoring its significance in promoting health and wellness.. Recent research extracted arabinoxylan (AX) from corn bran and fractionated it into AX-1–1, AX-2–1, and AX-3–1, which were purified and structurally analyzed.. These fractions had average molecular weights of 287, 305, and 280 kDa, respectively, and consisted mainly of arabinose and xylose, along with other saccharides, with A/X ratios of 0.78, 1.02, and 0.92.

    Imaging:

    Article Title: Oral KDS2010, a Monoamine Oxidase-B (MAO-B) Inhibitor, Slows the Deterioration of Motor Coordination in Genetic SCA1 Models by Inhibiting Astrocytic MAO-B-Mediated Inflammation.
    Article Snippet: The slides were incubated at 4°C for 24 h. After incubation, the slides were washed again with TBS buffer containing Triton X- 100 and incubated with the appropriate secondary antibodies (Anti- Rat IgG Alexa Fluor 488 (Invitrogen, cat. no. A21208), Anti- Rat IgG Alexa Fluor 633 (Invitrogen, cat. no. A21070), Anti- Mouse IgG Alexa Fluor 555 (Invitrogen, cat. no. A32727) and Anti- Rabbit IgG Alexa Fluor 568 (Invitrogen, cat. no. A- 11011)). .. During the third washing step, add DAPI (4′,6- diamidino- 2- phenylindole) (Santa Cruz, cat. no. sc- 373 715) to stain the nuclei, then mount the slides for imaging. .. During the third washing step, add DAPI (4′,6- diamidino- 2- phenylindole) (Santa Cruz, cat. no. sc- 373 715) to stain the nuclei, then mount the slides for imaging.



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    Image Search Results


    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing