dapi nuclear staining (Santa Cruz Biotechnology)
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Dapi Nuclear Staining, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+dapi/DAPI/pm42869916-314-14-20
Average 96 stars, based on 1459 article reviews
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Staining:Article Title: Single-nucleus multiomics of murine gonads reveals transcriptional regulatory network underlying supporting lineage differentiation Article Snippet: Specifically, RNAscope probes Mm-Lef1 (catalog no. 441861) and Mm-Msx1 (catalog no. 421841, Advanced Cell Diagnostics) were applied. .. Before Article Title: CD200 + fibroblasts prime the immunoregulatory function of granulocytic myeloid-derived suppressor cells in inflammatory arthritis. Article Snippet: .. Secondary antibodies were stained for 1 h at room temperature followed by incubation with Article Title: A cell-type-resolved human brain atlas of microRNAs and tRNA fragments Article Snippet: Cells were pelleted by centrifugation at 900 g for 5 minutes, resuspended in staining buffer, and incubated for 30 minutes with secondary antibodies for neurons (1:500; Alexa FluorTM 647 conjugated, Jackson ImmunoResearch, 711-605-152) and astrocytes (1:500; Cy3-conjugated; Jackson ImmunoResearch, 115-165-072). .. Cells were pelleted again as above and resuspended in Article Title: Macrophage-derived IL-6 reprograms lipid metabolism to promote colorectal cancer development through USP14-mediated FASN deubiquitination Article Snippet: For immunohistochemistry, the slices were incubated with antibodies against IL-6 (Cat#AF-206-NA, R&D Systems, Minneapolis, Minnesota, USA), p-STAT3 (Cell Signaling Technology, Danvers, Massachusetts, USA), USP14 (Proteintech, Wuhan, Hubei, China) and FASN (Cat#10624-2-AP, Proteintech) and then treated with an HRP-labeled secondary antibody (Proteintech). .. After the final round of staining, Article Title: Arabinoxylan derived from corn bran by-product: structural characterization and anti-inflammatory activity Article Snippet: 1 College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China 2 Key Laboratory of Traditional Chinese Korean Medicine Research (Yanbian University) of State Ethnic Affairs Commission, College of Pharmacy, Yanbian University, Yanji 133002, China 3 National Engineering Research Center for Wheat and Corn Deep Processing, Jilin Agricultural University, Changchun 130118, China Abstract Arabinoxylan, the preeminent constituent of hemicellulose B present in cereal bran, is widely recognized for its myriad physiological benefits, encompassing antioxidant, anticarcinogenic, immunomodulatory, intestinal protective, and prebiotic activities, thereby underscoring its significance in promoting health and wellness.. Recent research extracted arabinoxylan (AX) from corn bran and fractionated it into AX-1–1, AX-2–1, and AX-3–1, which were purified and structurally analyzed.. These fractions had average molecular weights of 287, 305, and 280 kDa, respectively, and consisted mainly of arabinose and xylose, along with other saccharides, with A/X ratios of 0.78, 1.02, and 0.92. Article Title: Glycine receptors in circulating white blood cells regulated by neuroinflammation Article Snippet: After incubation, the slides were washed three times with PBS for 5 minutes each, then incubated with appropriate secondary antibodies, i.e., Alexa Fluor 488 (1:200) and/or 594 (1:200) (Thermo-Fisher Scientific) for 1 hour at room temperature. .. A Article Title: Oral KDS2010, a Monoamine Oxidase-B (MAO-B) Inhibitor, Slows the Deterioration of Motor Coordination in Genetic SCA1 Models by Inhibiting Astrocytic MAO-B-Mediated Inflammation. Article Snippet: The slides were incubated at 4°C for 24 h. After incubation, the slides were washed again with TBS buffer containing Triton X- 100 and incubated with the appropriate secondary antibodies (Anti- Rat IgG Alexa Fluor 488 (Invitrogen, cat. no. A21208), Anti- Rat IgG Alexa Fluor 633 (Invitrogen, cat. no. A21070), Anti- Mouse IgG Alexa Fluor 555 (Invitrogen, cat. no. A32727) and Anti- Rabbit IgG Alexa Fluor 568 (Invitrogen, cat. no. A- 11011)). .. During the third washing step, add Blocking Assay:Article Title: Single-nucleus multiomics of murine gonads reveals transcriptional regulatory network underlying supporting lineage differentiation Article Snippet: Specifically, RNAscope probes Mm-Lef1 (catalog no. 441861) and Mm-Msx1 (catalog no. 421841, Advanced Cell Diagnostics) were applied. .. Before Incubation:Article Title: CD200 + fibroblasts prime the immunoregulatory function of granulocytic myeloid-derived suppressor cells in inflammatory arthritis. Article Snippet: .. Secondary antibodies were stained for 1 h at room temperature followed by incubation with Article Title: Neuroprotective Effects of Strength Training on Behavioral Deficit, Oxidative Damage, Astrogliosis, and Neuronal Death in a Bipolar Disorder Model Article Snippet: .. Sections were then incubated for 1 h in a solution containing 0.01% FJC (MilliporeSigma; cat. no. AG325) and 0.01% Fluorescence:Article Title: Arabinoxylan derived from corn bran by-product: structural characterization and anti-inflammatory activity Article Snippet: 1 College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China 2 Key Laboratory of Traditional Chinese Korean Medicine Research (Yanbian University) of State Ethnic Affairs Commission, College of Pharmacy, Yanbian University, Yanji 133002, China 3 National Engineering Research Center for Wheat and Corn Deep Processing, Jilin Agricultural University, Changchun 130118, China Abstract Arabinoxylan, the preeminent constituent of hemicellulose B present in cereal bran, is widely recognized for its myriad physiological benefits, encompassing antioxidant, anticarcinogenic, immunomodulatory, intestinal protective, and prebiotic activities, thereby underscoring its significance in promoting health and wellness.. Recent research extracted arabinoxylan (AX) from corn bran and fractionated it into AX-1–1, AX-2–1, and AX-3–1, which were purified and structurally analyzed.. These fractions had average molecular weights of 287, 305, and 280 kDa, respectively, and consisted mainly of arabinose and xylose, along with other saccharides, with A/X ratios of 0.78, 1.02, and 0.92. Microscopy:Article Title: Arabinoxylan derived from corn bran by-product: structural characterization and anti-inflammatory activity Article Snippet: 1 College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China 2 Key Laboratory of Traditional Chinese Korean Medicine Research (Yanbian University) of State Ethnic Affairs Commission, College of Pharmacy, Yanbian University, Yanji 133002, China 3 National Engineering Research Center for Wheat and Corn Deep Processing, Jilin Agricultural University, Changchun 130118, China Abstract Arabinoxylan, the preeminent constituent of hemicellulose B present in cereal bran, is widely recognized for its myriad physiological benefits, encompassing antioxidant, anticarcinogenic, immunomodulatory, intestinal protective, and prebiotic activities, thereby underscoring its significance in promoting health and wellness.. Recent research extracted arabinoxylan (AX) from corn bran and fractionated it into AX-1–1, AX-2–1, and AX-3–1, which were purified and structurally analyzed.. These fractions had average molecular weights of 287, 305, and 280 kDa, respectively, and consisted mainly of arabinose and xylose, along with other saccharides, with A/X ratios of 0.78, 1.02, and 0.92. Imaging:Article Title: Oral KDS2010, a Monoamine Oxidase-B (MAO-B) Inhibitor, Slows the Deterioration of Motor Coordination in Genetic SCA1 Models by Inhibiting Astrocytic MAO-B-Mediated Inflammation. Article Snippet: The slides were incubated at 4°C for 24 h. After incubation, the slides were washed again with TBS buffer containing Triton X- 100 and incubated with the appropriate secondary antibodies (Anti- Rat IgG Alexa Fluor 488 (Invitrogen, cat. no. A21208), Anti- Rat IgG Alexa Fluor 633 (Invitrogen, cat. no. A21070), Anti- Mouse IgG Alexa Fluor 555 (Invitrogen, cat. no. A32727) and Anti- Rabbit IgG Alexa Fluor 568 (Invitrogen, cat. no. A- 11011)). .. During the third washing step, add |
